In vitro suppression of spontaneous erythrocyte autoimmune responses with lymphocytes activated with concanavalin A

Journal Article · Sun Dec 31 23:00:00 EST 1978 · J. Immunol.; (United States)

OSTI ID:6434067

Ramshaw, I A; Woodsworth, M; Eidinger, D

When normal mouse spleen cells are cultured in vitro, large numbers of cells develop that produce antibody toward antigens found on bromelain-treated mouse erythrocytes (BrMRBC). The in vitro culture also generates T cells that mediate DTH toward these antigens. We have suggested that under in vivo conditions, suppressor T cells maintain these immune responses at a low level but that this suppression wanes when the cells are cultured in vitro. The present study examines the effect of concanavalin A (Con A) on the in vitro development of humoral and cell-mediated immunity to BrMRBC. Mitogenic concentrations of Con A prevented the development of both the PFC and T/sub DTH/ responses toward BrMRBC. The Con A-induced suppression was due to the induction of suppressor T cells; thus the addition of Con A-activated cells to fresh spleen cell cultures prevented the development of both the PFC and T/sub DTH/ response against BrMRBC.

• Research Organization: Univ. of Saskatchewan, Saskatoon
• OSTI ID: 6434067
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 122:1; ISSN JOIMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
ERYTHROCYTES
GLOBULINS
IMMUNE REACTIONS
IMMUNOSUPPRESSION
LEUKOCYTES
LYMPHOCYTES
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
SOMATIC CELLS
SPLEEN CELLS