Effect of dietary selenium concentration and duration of selenium feeding on hepatic glutathione concentrations in rats

Journal Article · Fri Nov 01 00:00:00 EST 1985 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-180-42187· OSTI ID:6425552

LeBoeuf, R A; Zentner, K L; Hoekstra, W G

Studies were conducted in rats to determine the effect of dietary selenium (Se) concentration on hepatic glutathione concentrations and enzyme activities associated with the maintenance of the cellular glutathione status. Male rats were fed 0.1, 3.0, or 6.0 ppm Se as Na/sub 2/SeO/sub 3/ for 2, 4, or 6 weeks at which time they were killed and analyses were performed. Both 3.0 and 6.0 ppm Se caused a significant dose-dependent increase in hepatic-reduced glutathione (GSH) by 4 weeks of feeding compared to 0.1 ppm Se. The increase in GSH was preceded by significant, dose-dependent increases in oxidized glutathione (GSSG) as well as the GSSG to GSH ratio. Increases in GSSG and the GSSG to GSH ratio as well as in glutathione reductase and glucose-6-phosphate dehydrogenase activities were observed by 2 weeks of high Se feeding. The current findings substantiate previous results demonstrating effects of high Se on hepatic glutathione concentrations and further suggest that increased cellular GSSG concentrations or the GSSG to GSH ratio caused by 3.0 and 6.0 ppm dietary Se signals for adaptive changes in hepatic glutathione metabolism.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 6425552
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Vol. 180:2, Issue 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
GLUTATHIONE
BIOCHEMICAL REACTION KINETICS
SELENIUM
BIOLOGICAL EFFECTS
DIET
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
EXPERIMENTAL DATA
INGESTION
LIVER
METABOLISM
OXIDOREDUCTASES
RATS
REDOX REACTIONS
ANIMALS
BODY
CHEMICAL REACTIONS
DATA
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
ENZYMES
GLANDS
INFORMATION
INTAKE
KINETICS
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RODENTS
SEMIMETALS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550200 - Biochemistry