Hepatic glutathione metabolism and lipid peroxidation in response to excess dietary selenomethionine and selenite in mallard ducklings. [Anas platyrhynchos]

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Journal of Toxicology and Environmental Health; (USA)

OSTI ID:5078447

Hoffman, D J; Heinz, G H; Krynitsky, A J ^[1]

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1. Research Center, Laurel, MD (USA)

Studies were conducted with mallard (Anas platyrhynchos) ducklings to determine the effects of excess dietary selenium (Se) on hepatic glatathione concentration and associated enzymes, and lipid peroxidation. Day-old ducklings were fed 0.1, 10, 20, or 40 ppm Se as seleno-DL-methionine or sodium selenite for 6 wk. Selenium from selenomethionine accumulated in a dose-dependent manner in the liver, resulting in a decrease in the concentration of hepatic-reduced glutathione (GSH) and total hepatic thiols (SH). These effects were accompanied by a dose-dependent increase in the ratio of oxidized glutathione (GSSG) to GSH, and an increase in malondialdehyde concentration as evidence of lipid peroxidation. Hepatic and plasma GSH peroxidase activity was initially elevated at 10 ppm Se as selenomethionine, whereas GSSG reductase activity was elevated at higher concentrations of Se. Selenium from sodium selenite accumulated in the liver to an apparent maximum at 10 ppm in the diet, resulting in an increase in hepatic GSH and GSSG accompanied by a small decrease in total hepatic SH. Sodium selenite resulted in an increase in hepatic GSSG reductase activity at 10 ppm and in plasm GSSG reductase activity at 40 ppm. A small increase in lipid peroxidation occurred at 40 ppm. These findings indicate that excess dietary Se as selenomethionine has a more pronounced effect on hepatic glutathione metabolism and lipid peroxidation in ducklings than dose selenite, which may be related to the pattern of accumulation. Effects of Se as selenite appear to be less pronounced in ducklings than reported in laboratory rodents. The effects of selenomethionine, which occurs in vegetation, are of particular interest with respect to the health of wild aquatic birds in seleniferous locations.

• OSTI ID: 5078447
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Journal Name: Journal of Toxicology and Environmental Health; (USA) Vol. 27:2; ISSN JTEHD; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
DUCKS
ELEMENTS
ENZYMES
FOWL
GLANDS
GLUTATHIONE
INGESTION
INTAKE
LIPIDS
LIVER
MATERIALS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDATION
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
SELENIUM
SELENIUM COMPOUNDS
SEMIMETALS
THIOLS
TOXICITY
VERTEBRATES