In vivo assessment of phagocytic properties of Kupffer cells
Three-compartment analysis was used to assess the kinetics of phagocytosis of Tc-99m-labeled human serum albumin microparticles (Tc-99m HSA-MM) in human Kupffer cells in vivo. The tracer turnover in these phagocytic cells could be described by a monoexponential accumulation with a two-stage elimination phase. Three-compartment analysis of the Tc-99m HSA-MM kinetics allowed us to quantify tracer attachment, phagocytosis, and degradation in Kupffer cells. The calculated time course of phagocytosis in ten control subjects proved to be identicl to that of phagocytosis of various test substances in mouse macrophage monolayers. In addition, an impairment of particle turnover at the macrophage membrane, a significantly diminished phagocytosis rate of the tracer, was observed in ten patients with various tumors.
- Research Organization:
- Nuclear Research Center, Juelich, Germany
- OSTI ID:
- 6411400
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 22:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
DRUGS
DYNAMIC FUNCTION STUDIES
ELECTRON MICROSCOPY
GLANDS
HOURS LIVING RADIOISOTOPES
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIVER
MACROPHAGES
MAMMALS
MAN
MICE
MICROSCOPY
MICROSPHERES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PHAGOCYTES
PHAGOCYTOSIS
PRIMATES
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
RODENTS
SCANNING ELECTRON MICROSCOPY
SOMATIC CELLS
TECHNETIUM 99
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES