In vivo assessment of phagocytic properties of Kupffer cells
Three-compartment analysis was used to assess the kinetics of phagocytosis of Tc-99m-labeled human serum albumin microparticles (Tc-99m HSA-MM) in human Kupffer cells in vivo. The tracer turnover in these phagocytic cells could be described by a monoexponential accumulation with a two-stage elimination phase. Three-compartment analysis of the Tc-99m HSA-MM kinetics allowed us to quantify tracer attachment, phagocytosis, and degradation in Kupffer cells. The calculated time course of phagocytosis in ten control subjects proved to be identical to that of phagocytosis of various test substances in mouse macrophage monolayers (1). In addition, an impairment of particle turnover at the macrophage membrane, a significantly diminished (p less than 0.01) phagocytosis rate of the tracer, was observed in ten patients with various tumors.
- OSTI ID:
- 5579037
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 22:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
HOURS LIVING RADIOISOTOPES
IMMUNE SERUMS
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PHAGOCYTOSIS
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
REACTION KINETICS
RETICULOENDOTHELIAL SYSTEM
TECHNETIUM 99
TECHNETIUM ISOTOPES
TIME DEPENDENCE
TISSUES
YEARS LIVING RADIOISOTOPES