Cadmium and zinc binding in mammalian liver and kidneys

Journal Article · Thu Jun 01 04:00:00 EDT 1972 · Arch. Environ. Health; (United States)

DOI:https://doi.org/10.1080/00039896.1972.10666118· OSTI ID:6403268

Shaikh, Z A; Lucis, O J

Rats and mice were injected subcutaneously with cadmium chloride Cd 109 and zinc chloride Zn 65. Both isotopes concentrated primarily in liver and kidneys. Tissue subcellular fractionation showed 70% to 80% /sup 109/Cd and /sup 65/Zn associated with cytoplasmic soluble fraction. Soluble fraction gel filtration revealed most /sup 109/Cd was bound to proteins of 11,000 to 12,000 molecular weight; /sup 65/Zn was associated mainly with larger sized molecules. In rats, two weeks following isotope dose, depletion of /sup 65/Zn from cytoplasmic macromolecules was demonstrated; /sup 109/Cd remained fixed to cadmium-binding proteins (Cd-BP). By ion-exchange chromatography, Cd-BP was resolved into major components. Similar components were separated from human kidney Cd-BP. Presence of Cd-BP was noted in humans and monkey liver tissues. It is suggested that mammalian organism intracellular Cd-BP apparently acts as biochemical mechanism for sequestration of toxic Cd/sup 2 +/ ions.

• Research Organization: Dalhousie Univ., Halifax, Nova Scotia
• OSTI ID: 6403268
• Journal Information: Arch. Environ. Health; (United States), Journal Name: Arch. Environ. Health; (United States) Vol. 24:6; ISSN AEHLA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL PATHWAYS
BODY
CADMIUM
DIGESTIVE SYSTEM
ELEMENTS
GLANDS
KIDNEYS
LIVER
MAMMALS
METALS
MICE
ORGANS
RATS
RODENTS
VERTEBRATES
ZINC