Improved mutagen-testing systems in mice: Progress report, June 1, 1988--May 31, 1989
In December we injected 20 mice with 250 mg/kg of ENU. Half of the mice were males of C57BL/6J and half were males of strains AEJ/GnRk. The strains were chosen to include a standard widely used strain (C57BL/6J) but also to include a strain (AEJ/GnRk) that has an unusually large litter size (mean = 10) to promote better survival of embryos with induced recessives. Sentinal females are in with the treated males to determine return to fertility of the injected males. All males will be mated with the new lethal test system. Lethal No. 1 has now been clearly determined to be just distal to the distal breakpoint of inversion In(1)1Rk. All other lethals, including the new lethal No. 8 are crossed with our specially constructed stocks for linkage analysis. Also, the lethals are being crossed with known recessive markers along the inverted segment to determine possible allelism. All lethals have been intercrossed to determine whether they complement each other. Wild type animals should not be recovered if the lethals are overlapping. In a new study we are examining the wild type animals from each of these crosses carefully for general phenotypic conformation, eye disorders, feet, body weight and gait. Although these lethals may be complementary, it is possible that some interactive effects could be produced in an animal doubly heterozygous for different deletions.
- Research Organization:
- Jackson Lab., Bar Harbor, ME (USA)
- DOE Contract Number:
- FG02-88ER60697
- OSTI ID:
- 6397420
- Report Number(s):
- DOE/ER/60697-1; ON: DE89006987
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL PATHWAYS
CELL TRANSFORMATIONS
DOCUMENT TYPES
GENES
GENETIC MAPPING
MAMMALS
MAPPING
MICE
MOLECULAR BIOLOGY
MUTAGEN SCREENING
MUTAGENS
MUTATIONS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
OPTIMIZATION
PHENOTYPE
PROGRESS REPORT
RODENTS
SCREENING
SOMATIC MUTATIONS
VERTEBRATES