Identification of fentanyl metabolites in rat urine by gas chromatography-mass spectrometry with stable-isotope tracers
The metabolites of fentanyl (l), which has been widely used as a neuroleptic analgesic agent, were identified in urine of rats by gas chromatography-mass spectrometry combined with a stable-isotope tracer technique. After the oral administration of an equimolar mixture of l and deuterium-labeled l (l/l-d5), the urinary metabolites were extracted with chloroform at pH 9.0. Extracts were derivatized and analyzed by GC/MS. Metabolites were identified by the presence of doublet ion peaks separated by 5 amu, and chemical structures were established from analyses of fragmentation pathways. The metabolites were identified as 4-N-(N-propionylanilino)-piperidine, 4-N-(N-hydroxypropionylanilino)piperidine, 4-N-(N-propionylanilino) hydroxypiperidine, 1-(2-phenethyl)-4-N-(N-hydroxypropionylanilino)piperidine and 1-(2-phenethyl)-4-N-(N-propionylanilino)hydroxypiperidine. These metabolites, together with unchanged l, were also detected in urine of rats receiving l/l-d5 intravenously, by selected-ion monitoring of the specific cluster ions.
- Research Organization:
- Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan
- OSTI ID:
- 6374065
- Journal Information:
- Drug Metab. Dispos.; (United States), Vol. 10:5
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
ANALGESICS
METABOLISM
PHARMACOLOGY
METABOLITES
QUALITATIVE CHEMICAL ANALYSIS
NARCOTICS
DEUTERIUM COMPOUNDS
RATS
URINE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL ANALYSIS
DRUGS
HYDROGEN COMPOUNDS
MAMMALS
MATERIALS
RODENTS
VERTEBRATES
WASTES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550601 - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques