Platelet affinity for burro aorta collagen

Journal Article · Sat Oct 01 04:00:00 EDT 1977 · Am. J. Vet. Res.; (United States)

OSTI ID:6369111

Schneider, M D

Despite ingenious concepts, there are no unequivocal clues as to what, when, and how some undefined biochemical factor(s) or constituent(s) that localizes in the arterial wall can precipitate a thromboatheromatous lesion or arterial disease. The present study focused on the extraction, partial purification, and characterization of a collagen-active platelet stimulator from the aortas of aged burros. The aggregator moiety in the aorta extracts invariably had a higher affinity for platelets in citrated platelet-rich plasma of human beings than for platelets of homologous burros. The platelet-aggregating factor(s) in the aorta extract was retained by incubation with ..cap alpha..-chymotrypsin. Platelet-aggregating activity was rapidly abolished after incubation with collagenase, as determined by platelet-aggregometry tests. Evidence based on light microscope and polysaccharide histochemical reactions indicates a probability that the intracellular amorphous matrix (PAS-positive) and filamentous components (PTAH-positive) expelled from smooth muscle cells disrupted during homogenization of the aorta may be a principal source of a precursor collagen species which is a potent inducer of platelet aggregation.

• Research Organization: Comparative Animal Research Lab., Oak Ridge, TN
• OSTI ID: 6369111
• Journal Information: Am. J. Vet. Res.; (United States), Journal Name: Am. J. Vet. Res.; (United States) Vol. 38:10; ISSN AJVRA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
AORTA
ARTERIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
BURROS
CARDIOVASCULAR SYSTEM
COLLAGEN
KINETICS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
SCLEROPROTEINS
VERTEBRATES