Burro aortic collagen: platelet aggregating activity and ultrastructural changes induced by plasma

Journal Article · Wed Mar 01 04:00:00 EST 1978 · Am. J. Vet. Res.; (United States)

OSTI ID:6550219

Schneider, M D ^[1]; Cross, F H; Dumont, J N

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1. Comparative Animal Research Lab., Oak Ridge, TN

A fibrillar collagen molecule was extracted from the upper thoracic aorta of an old burro (Equus asinus). Presence of the collagen in the extract was determined by amino acid analysis, scanning and transmission electron microscopy, incubation with collagenase, and assays of its platelet-aggregating capacity by ''aggregometry.'' Based on the amino acid ratios of proline/hydroxyproline and lysine/hydroxylysine, the collagenous protein most nearly resembles type I of 4 main published types of collagen. Quantitative assays of the collagen as a mediator of platelet aggregation showed human platelets more sensitive and sheep platelets slightly less sensitive than burro platelets. Incubation with collagenase abolished platelet aggregation capacity and converted the fibrillar collagen to a gel-like mass. Incubation with galactose oxidase neither lessened nor intensified the collagen-mediated platelet aggregation. Incubation with burro plasma decreased platelet aggregating activity and changed the collagen ultrastructure (demonstrated with scanning electron microscopic imaging). The significance of a naturally occurring plasma (protein) factor(s) which may have a regulatory role in reducing the chemical activity of the fibrillar collagen molecule with platelets is also discussed.

• OSTI ID: 6550219
• Journal Information: Am. J. Vet. Res.; (United States), Journal Name: Am. J. Vet. Res.; (United States) Vol. 39:3; ISSN AJVRA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AORTA
ARTERIES
BEHAVIOR
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLASMA
BLOOD PLATELETS
BLOOD VESSELS
BODY FLUIDS
BURROS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
COLLAGEN
ELECTRON MICROSCOPY
ENZYMES
MAMMALS
MICROSCOPY
MORPHOLOGICAL CHANGES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
SCLEROPROTEINS
ULTRASTRUCTURAL CHANGES
VERTEBRATES