Need to develop a short-term Chinese hamster in-vivo specific-locus mutational assay

Conference · Fri Jan 01 04:00:00 EST 1982

OSTI ID:6338869

Hsie, A W; Schenley, R L

This paper summaries mutagenesis studies using Chinese hamster ovary (CHO) cells and briefly explains the use of CHO cells for studies of mutagenic effects at the cytogenetic level including mutagen-induced changes in DNA nucleotide sequences. The existence of a large data base on gene mutation, chromosome aberration, and sister chromatid exchange in cultured Chinese hamster cells argue favorably for the need to develop an in vivo assay system. Since CHO cells in vitro are incapable of converting promutagens to mutagenic forms except when coupled with a metabolic activation system, a short-term specific locus mutation assay in vivo using the Chinese hamster as a model animal is proposed. (ACR)

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6338869
• Report Number(s): CONF-820448-5; ON: DE83010719
• Country of Publication: United States
• Language: English

Related Subjects

560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MODELS
BODY
CELL CULTURES
CHROMOSOMAL ABERRATIONS
DNA
FEMALE GENITALS
GENE MUTATIONS
GONADS
HAMSTERS
IN VITRO
IN VIVO
MAMMALS
MUTAGEN SCREENING
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
RESEARCH PROGRAMS
RODENTS
SCREENING
SISTER CHROMATID EXCHANGES
VERTEBRATES