Effect of glutathione depletion on UCO2 evolution from ( UC)methyl-labeled aminopyrine in intact rats

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Hepatol.; (United States)

DOI:https://doi.org/10.1002/hep.1840050416· OSTI ID:6335612

Bhatt, H S; Combes, B

The effect of hepatic glutathione depletion on UCO2 evolution from ( UC)methyl-labeled aminopyrine was assessed in fed male Sprague-Dawley rats. Within 30 min of i.p. administration of either diethylmaleate or phorone, hepatic glutathione fell approximately 75 to 80% and remained depressed for the ensuing 120 min. ( UC)Aminopyrine was i.p. administered 30 min after the glutathione-lowering agents (zero time) and exhaled UCO2 was collected at 15-min intervals for the next 120 min. Parameters of UCO2 exhalation including peak exhalation rate, cumulative exhalation from 0 to 120 min and the elimination rate constant were all impaired in glutathione-depleted rats. Metabolism of the ( UC)methyl groups involves N-demethylation with formation of formaldehyde, oxidation to formate and conversion to UCO2. Glutathione depletion did not affect CO2 evolution from i.p. administered formate or bicarbonate. The glutathione-dependent step presumably involves either or both generation of formaldehyde or its subsequent oxidation to formate.

• Research Organization: Univ. of Texas Health Science Center, Dallas
• OSTI ID: 6335612
• Journal Information: Hepatol.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
METABOLISM
CARBON DIOXIDE
EXHALATION
ORGANIC NITROGEN COMPOUNDS
GLUTATHIONE
INHIBITION
KETONES
LIVER
RATS
SOLVENTS
ANIMALS
BODY
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CLEARANCE
DIGESTIVE SYSTEM
DRUGS
EXCRETION
GLANDS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques