Recovery of liver function in partially hepatectomized rats evaluated by aminopyrine demethylation capacity

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · Hepatol.; (United States)

DOI:https://doi.org/10.1002/hep.1840050418· OSTI ID:6287406

Sendama, I; de Hemptinne, B; Lambotte, L

Aminopyrine demethylation was investigated in rats after a 70% hepatectomy to assess possible parallelism between the recovery of mass and function. Tests were performed by analyzing UCO2 exhalation from 0.1 microCi per 100 gm of body weight of (dimethylamine- UC)aminopyrine given intraperitoneally with incremental doses of unlabeled drug. Early after 70% hepatectomy, Vmax was reduced by 52%. This discordance between mass and function was not due to extrahepatic aminopyrine demethylation, since liver exclusion reduced demethylation of aminopyrine to nearly nil. Whether it results from increased liver blood flow in the remnant liver is less clear. The early increase in Vmax could be related to a hepatotrophic factor of splanchnic origin which increased after partial hepatectomy and decreased after portacaval shunt. After the early period, Vmax, expressed per gram of actual liver weight, returned to control range. Throughout regeneration (4 to 144 hr), no modification was observed in Km nor in cytochrome P-450 concentration. Enzymatic induction with phenobarbital increased the demethylation capacity more than liver weight in intact and regenerating liver. Except for the first hours after partial hepatectomy or after enzymatic induction, the aminopyrine demethylation capacity directly correlated with liver mass and may be useful in evaluating liver regeneration in vivo.

• Research Organization: Univ. of Louvain, Brussels, Belgium
• OSTI ID: 6287406
• Journal Information: Hepatol.; (United States), Journal Name: Hepatol.; (United States) Vol. 4; ISSN HPTLD
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL FUNCTIONS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
CLEARANCE
DIGESTIVE SYSTEM
ENZYMES
EXCRETION
EXHALATION
FUNCTIONS
GLANDS
HEPATECTOMY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEDICINE
MIXED-FUNCTION OXIDASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
RATS
RODENTS
SURGERY
TRACER TECHNIQUES
VERTEBRATES