Impaired lipid accumulation in the liver of Tsc2-heterozygous mice during liver regeneration

Journal Article · Fri Jul 19 04:00:00 EDT 2013 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2013.06.056· OSTI ID:22239677

Obayashi, Yoko ^[1]; Campbell, Jean S.; Fausto, Nelson ^[1]; Yeung, Raymond S. ^[2]

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1. Department of Pathology, University of Washington School of Medicine, Seattle, WA (United States)
2. Department of Surgery, University of Washington School of Medicine, Seattle, WA (United States)

Highlights: •Tuberin phosphorylation correlated with mTOR activation in early liver regeneration. •Liver regeneration in the Tsc2+/− mice was not enhanced. •The Tsc2+/− livers failed to accumulate lipid bodies during liver regeneration. •Mortality rate increased in Tsc2+/− mice after partial hepatectomy. •Tuberin plays a critical role in hepatic lipid accumulation to support regeneration. -- Abstract: Tuberin is a negative regulator of mTOR pathway. To investigate the function of tuberin during liver regeneration, we performed 70% hepatectomy on wild-type and Tsc2+/− mice. We found the tuberin phosphorylation correlated with mTOR activation during early liver regeneration in wild-type mice. However, liver regeneration in the Tsc2+/− mice was not enhanced. Instead, the Tsc2+/− livers failed to accumulate lipid bodies, and this was accompanied by increased mortality. These findings suggest that tuberin plays a critical role in liver energy balance by regulating hepatocellular lipid accumulation during early liver regeneration. These effects may influence the role of mTORC1 on cell growth and proliferation.

• OSTI ID: 22239677
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 437; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BRAIN
ENERGY BALANCE
FLUORIDES
HEPATECTOMY
LIPIDS
LIVER
MICE
MORTALITY
PH VALUE
PHOSPHORYLATION
PROTEINS