Binding assays for the quantitative detection of P. brevis polyether neurotoxins in biological samples and antibodies as therapeutic aids for polyether marine intoxication. Annual report, 1 December 1987-30 November 1988

Technical Report · Thu Dec 15 04:00:00 EST 1988

OSTI ID:6325214

Baden, D G

The polyether lipid-soluble toxins isolated from the marine dinoflagellate Ptychodiscus brevis (formerly Gymnodinium breve) can be detected using two separate types of specific binding reaction. Using tritiated PbTx-3 as a specific probe for binding to voltage-dependent sodium channels in rat brain synaptosomes or to specific polyclonal antibodies, binding equilibria and displacement by unlabeled brevetoxins were compared. Labeled toxin can be displaced in a competitive manner by any of the other 5 naturally-occurring toxins; the quantitative displacement ability of each appears to reflect individual potency in fish bioassay. A comparison of ED50 in Radioimmunoassay and ED50 in synaptosome binding assay indicates that the former assay is useful for detection of toxins which possess the structural backbone of PbTx-3, the immunizing hapten. Thus, the two assays have quantitative applicability; the sodium channel with respect to potency and the antibodies with respect to structure. Microtiter plate assays utilizing each specific brevetoxin binding component and enzyme-linked toxin hapten have been successful and indicate a general applicability of colorimetric prototypes. There, is however, considerable manipulation required to decrease non-specific binding of the hydrophobic toxin-enzyme complex to the plates. Preliminary studies aimed at producing monoclonal antibodies have been explored using brevetoxins linked to keyhole limpet hemocyanin.

• Research Organization: Miami Univ., Coral Gables, FL (USA)
• OSTI ID: 6325214
• Report Number(s): AD-A-203487/4/XAB
• Country of Publication: United States
• Language: English

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ALKALI METALS
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
DETECTION
DOCUMENT TYPES
ELEMENTS
FISHES
HYDROGEN ISOTOPES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
METALS
NERVE CELLS
NUCLEI
ODD-EVEN NUCLEI
POLLUTION
PROBES
PROGRESS REPORT
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SAMPLING
SODIUM
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
WATER POLLUTION
YEARS LIVING RADIOISOTOPES