Development of an antibody that binds sulfur mustard. (Reannouncement with new availability information)

Technical Report · Thu Dec 31 04:00:00 EST 1992

OSTI ID:166869

Lieske, C N; Klopcic, R S; Gross, C L; Clark, J H; Dolzine, T W

An antibody that binds bis(2-chloroethyl)sulfide (sulfur mustard) was developed. The immunizing antigen was prepared from the hapten 4-(2-chloroethyl)benzoic acid covalently bound to keyhole limpet hemocyanin (KLH). The antibody was monitored by a solid phase enzyme-linked immunosorbent assay (ELISA). The test antigen consisted of a second hapten, 8-chlorocaprylic acid, covalently bound to bovine serum albumin (BSA). The test antigen was absorbed to the wells of 96-well plates. The immunizing and test antigens contain a common chloroethyl moiety. Thiodiglycol, the principal hydrolysis product of sulfur mustard, does not react with the antibody. This antibody, because of its specificity, has the potential to be a valuable tool for mustard research and forensic detection. Sulfur mustard, sulfur mustard antibody, antibody inhibition haptens.

• Research Organization: Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (United States)
• OSTI ID: 166869
• Report Number(s): AD-A--250743/2/XAB; USAMRICD-P--91-005
• Country of Publication: United States
• Language: English

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANTIBODIES
ANTIGENS
BENZOIC ACID
CHEMICAL WARFARE AGENTS
HEMOCYANIN
PREVENTIVE MEDICINE
THERAPEUTIC USES
TOXICITY