Development of an antibody that binds sulfur mustard. (Reannouncement with new availability information)
An antibody that binds bis(2-chloroethyl)sulfide (sulfur mustard) was developed. The immunizing antigen was prepared from the hapten 4-(2-chloroethyl)benzoic acid covalently bound to keyhole limpet hemocyanin (KLH). The antibody was monitored by a solid phase enzyme-linked immunosorbent assay (ELISA). The test antigen consisted of a second hapten, 8-chlorocaprylic acid, covalently bound to bovine serum albumin (BSA). The test antigen was absorbed to the wells of 96-well plates. The immunizing and test antigens contain a common chloroethyl moiety. Thiodiglycol, the principal hydrolysis product of sulfur mustard, does not react with the antibody. This antibody, because of its specificity, has the potential to be a valuable tool for mustard research and forensic detection. Sulfur mustard, sulfur mustard antibody, antibody inhibition haptens.
- Research Organization:
- Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (United States)
- OSTI ID:
- 166869
- Report Number(s):
- AD-A--250743/2/XAB; USAMRICD-P--91-005
- Country of Publication:
- United States
- Language:
- English
Similar Records
Enzyme-linked immunosorbent assay for the specific detection of the mercapturic acid metabolites of naphthalene
Role of lipid in the induction of hapten-specific delayed hypersensitivity and contact sensitivity