Normal rejoining of DNA strand breaks in ataxia telangiectasia fibroblast lines after low x-ray exposure
Journal Article
·
· Radiat. Res.; (United States)
- Roswell Park Memorial Inst., Buffalo, NY
The alkaline elution method was used to measure the enzymatic repair of x-ray-induced DNA strand breaks in skin fibroblasts derived from human subjects afflicted with ataxia telangiectasia (AT). Monolayer cultures of normal control and AT cell lines were exposed acutely to moderately lethal (250-rad) and highly lethal (1250-rad) doses of 250-kV x rays under aerobic conditions. Upon receiving 250 rad, the control fibroblasts from a clinically normal donor rejoined all detectable single-strand breaks (plus alkali-labile bonds) within 30 to 60 min of incubation. When challenged with 1250 rad the kinetics of strand rejoining by the normal control cells were biphasic. For both exposures, no significant difference in either the rate or the extent of strand rejoining was detected between the normal cell line (GM38) and three mutant cell lines (AT2BE, AT3BI, AT4BI) belonging to the three known genetic complementation groups in AT. It would thus appear that the enhanced radiosensitivity of cultured AT cells does not stem from faulty rejoining of radiogenic DNA strand breaks.
- OSTI ID:
- 6318365
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 86:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rejoining of DNA double-strand breaks in human fibroblasts and its impairment in one ataxia telangiectasia and two Fanconi strains
Effects of hyperthermia and ionizing radiation in normal and ataxia telangiectasia human fibroblast lines
Quantitative measurement of DNA strand breaks and repair in. gamma. -irradiated human leukocytes from normal and ataxia telangiectasia donors
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· J. Supramol. Struct. Cell. Biochem.; (United States)
·
OSTI ID:5239589
Effects of hyperthermia and ionizing radiation in normal and ataxia telangiectasia human fibroblast lines
Journal Article
·
Sat Sep 01 00:00:00 EDT 1984
· Radiat. Res.; (United States)
·
OSTI ID:6411967
Quantitative measurement of DNA strand breaks and repair in. gamma. -irradiated human leukocytes from normal and ataxia telangiectasia donors
Journal Article
·
Sat Jun 01 00:00:00 EDT 1985
· Radiat. Res.; (United States)
·
OSTI ID:5642457
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
IONIZING RADIATIONS
IRRADIATION
LOW DOSE IRRADIATION
MAMMALS
MAN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
SKIN
SKIN DISEASES
SOMATIC CELLS
STRAND BREAKS
TELANGIECTASIS
VASCULAR DISEASES
VERTEBRATES
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
IONIZING RADIATIONS
IRRADIATION
LOW DOSE IRRADIATION
MAMMALS
MAN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
SKIN
SKIN DISEASES
SOMATIC CELLS
STRAND BREAKS
TELANGIECTASIS
VASCULAR DISEASES
VERTEBRATES
X RADIATION