Quantitative measurement of DNA strand breaks and repair in. gamma. -irradiated human leukocytes from normal and ataxia telangiectasia donors

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576710· OSTI ID:5642457

Thierry, D; Rigaud, O; Duranton, I; Moustacchi, E; Magdelenat, H

Fluorimetric analysis of DNA unwinding, which allows measurement of DNA strand breaks in human leukocytes, has been optimized by reducing the amount of cells required for the test and by modifying the DNA alkali unwinding conditions. The permitted measurement of DNA strand-break induction in cells irradiated with low (0.5-7 Gy) or high doses (5-20 Gy) of ..gamma.. rays. Linear dose-response curves were obtained for both dose ranges. Presence of cysteamine during irradiation caused a decrease in the extent of DNA strand breaks. The kinetics of the DNA standard-break rejoining process appeared to be biphasic over the dose range of 2-20 Gy when plotted on a linear vs linear axis (percentage of damage as a function of time). No difference in the level of DNA strand breaks and the rate of repair of these breaks was observed between leukocytes from three ataxia telangiectasia patients and those from normal donors.

• Research Organization: Institut Curie, Paris, France
• OSTI ID: 5642457
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CESIUM 137
CESIUM ISOTOPES
DISEASES
DNA
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
GAMMA RADIATION
IONIZING RADIATIONS
ISOTOPES
LEUKOCYTES
MATERIALS
MEA
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SKIN DISEASES
SPECTROSCOPY
STRAND BREAKS
TELANGIECTASIS
THIOLS
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES