The response of ataxia-telangiectasia lymphoblastoid cells to neutron irradiation

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3578110· OSTI ID:6057041

Houldsworth, J; Cohen, D; Singh, S; Lavin, M F ^[1]

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1. Sloan-Kettering Cancer Center, New York, NY (USA)

The response of control and ataxia-telangiectasia (A-T) cells to increasing doses of high-linear-energy-transfer (LET) ionizing radiation (neutrons) was compared. Ataxia-telangiectasia cells were markedly more sensitive to neutron irradiation than were control cells. The D0 value for the two A-T cell lines was 0.4 Gy while the value for controls was approximately 1.4 Gy. Fast neutrons were considerably more effective than gamma rays in inducing cell death in both cell types, but the sensitivity factor remained approximately the same as with gamma rays. A minimal depression of DNA synthesis was observed in ataxia-telangiectasia cells after neutron irradiation, similar to that reported previously after gamma irradiation. The extent of inhibition was not significantly greater in control cells, contrary to that seen with gamma rays. In time-course experiments a significant difference in degree of inhibition of DNA synthesis was observed between the cell types. Low doses of fast neutrons induced a G2-phase delay in both cell types, but the degree and extent of this delay was greater in ataxia-telangiectasia cells as observed previously with low-LET radiation.

• OSTI ID: 6057041
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 125:3; ISSN RAREA; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
DISEASES
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELEMENTARY PARTICLES
ENERGY TRANSFER
FAST NEUTRONS
FERMIONS
HADRONS
LET
MAMMALS
MAN
MEDICINE
NEUTRONS
NUCLEAR MEDICINE
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PRIMATES
RADIATION DOSES
RADIATION EFFECTS
RADIOLOGY
RADIOSENSITIVITY
RADIOTHERAPY
RBE
SKIN DISEASES
TELANGIECTASIS
THERAPY
TUMOR CELLS
VASCULAR DISEASES
VERTEBRATES