The effect of bleomycin on DNA synthesis in ataxia telangiectasia lymphoid cells
Bleomycin, a radiomimetic glycopeptide, inhibits de novo DNA synthesis in ataxia telangiectasia lymphoblastoid B cells to a markedly lesser extent than in normal and xeroderma pigmentosum lymphoid cells. This observation is similar to that following ionizing radiation; however, the effect is slower following the chemical treatment. Recovery of the normal cells occurs 15-18 hours after treatment, whereas the ataxia telangiectasia lines do not attain normal levels of DNA synthesis during the entire 24-hour observation period. Similar differences were not observed following treatment with mitomycin C, a bifunctional alkylating agent, indicating a specific effect of bleomycin on DNA synthesis in ataxia telangiectasia cells. Following bleomycin treatment and preincubation with hydroxyurea, residual DNA synthesis in ataxia telangiectasia cells was similar to that in both normal and xeroderma pigmentosum lymphoid lines, suggesting that the capacity to repair the induced DNA lesion is present.
- Research Organization:
- Northwestern Univ. Medical School, Chicago
- OSTI ID:
- 5440069
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 4:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BLEOMYCIN
BIOLOGICAL EFFECTS
DNA
INHIBITION
BIOLOGICAL REPAIR
CELL CULTURES
HEREDITARY DISEASES
LYMPHOCYTES
PATIENTS
RADIOMIMETIC DRUGS
RADIOSENSITIVITY EFFECTS
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
LEUKOCYTES
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RECOVERY
REPAIR
SOMATIC CELLS
560171* - Radiation Effects- Nuclide Kinetics & Toxicology- Man- (-1987)