The effect of bleomycin on DNA synthesis in ataxia telangiectasia lymphoid cells
Journal Article
·
· Environ. Mutagen.; (United States)
Bleomycin, a radiomimetic glycopeptide, inhibits de novo DNA synthesis in ataxia telangiectasia lymphoblastoid B cells to a markedly lesser extent than in normal and xeroderma pigmentosum lymphoid cells. This observation is similar to that following ionizing radiation; however, the effect is slower following the chemical treatment. Recovery of the normal cells occurs 15-18 hours after treatment, whereas the ataxia telangiectasia lines do not attain normal levels of DNA synthesis during the entire 24-hour observation period. Similar differences were not observed following treatment with mitomycin C, a bifunctional alkylating agent, indicating a specific effect of bleomycin on DNA synthesis in ataxia telangiectasia cells. Following bleomycin treatment and preincubation with hydroxyurea, residual DNA synthesis in ataxia telangiectasia cells was similar to that in both normal and xeroderma pigmentosum lymphoid lines, suggesting that the capacity to repair the induced DNA lesion is present.
- Research Organization:
- Northwestern Univ. Medical School, Chicago
- OSTI ID:
- 5440069
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4:1; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLEOMYCIN
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DRUGS
HEREDITARY DISEASES
INHIBITION
LEUKOCYTES
LYMPHOCYTES
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
RADIOMIMETIC DRUGS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLEOMYCIN
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DRUGS
HEREDITARY DISEASES
INHIBITION
LEUKOCYTES
LYMPHOCYTES
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
RADIOMIMETIC DRUGS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS