Mechanisms for radiation damage in DNA. Progress report, January 1, 1981-December 31, 1981

Technical Report · Tue Sep 01 04:00:00 EDT 1981

OSTI ID:6314983

Sevilla, M D

Several mechanisms for radiation damage to DNA constituents and DNA are proposed, and a series of experiments utilizing electron spin resonance spectrometry to test the proposed mechanisms is detailed. Past experiments have concentrated chiefly on the direct affect of radiation on DNA. Currently irradiated systems on DNA constituents are being investigated to shed light on indirect effects. In addition, studies of radiation effects on lipids were undertaken which will shed light on the only other proposed site for cell kill, the membrane. Studies which were completed or progressed during the past year include: (1) study of the oxidation radicals in azauracil and azathymine: a case for both sigma and ..pi.. radicals; (2) studies of the ..pi..-cation radicals of the 5-halouracils; (3) hydrogen abstraction reactions by the peptide bond protonated anion: a comparison to acid, ester, aldehyde, and ketone protonated anions; (4) internal hydrogen bonding in the ..pi..-cations of hydroxymethyluracil and hydroxymethyl-cytosine; and (5) study of radicals generated in model compounds for the sugar-phosphate backbone.

• Research Organization: Oakland Univ., Rochester, MI (USA)
• DOE Contract Number: AS02-76EV02364
• OSTI ID: 6314983
• Report Number(s): DOE/EV/02364-T2; COO-2364-19; ON: DE81026637
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL KILLING
CELL MEMBRANES
DNA
IONIZING RADIATIONS
LIPIDS
MEMBRANES
MOLECULAR BIOLOGY
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOINDUCTION