Mechanisms for radiation damage in DNA. Progress report, January 1, 1982-December 31, 1982
In this project several mechanisms are proposed for radiation damage to DNA constituents and DNA, and a series of experiments utilizing electron spin resonance spectrometry to test the proposed mechanisms have been detailed. In the past studies have concentrated chiefly on the direct effect of radiation on DNA. We are currently investigating irradiated systems of DNA constituents which may shed light on indirect effects. In addition, studies of radiation effects on lipids have been undertaken which will shed light on the only other proposed site for cell kill, the membrane. Studies which we have completed during this year are: (1) ESR Study of Radicals Produced by One Electron Loss from 6-Azauracil, 6-Azathymine and 6-Azacytosine: Evidence for Both sigma and ..pi..-Radicals; (2) Hydrogen Abstraction Reactions by Amide Electron Adducts: A Comparison to Acid, Ester, Aldehyde and Ketone Electron Adducts. Studies which we have made progress on are: (3) An ESR and INDO Study of the ..pi..-Cations of 5-Hydroxymethyl-uracil and 5-Hydroxymethyl-cytosine: Evidence for Intramolecular Hydrogen Bonding; (4) Studies of the ..pi..-Cations of 5-Halouracils and 5-Halocytosines; (5) Studies of Radiation Damage to Lipids.
- Research Organization:
- Oakland Univ., Rochester, MI (USA)
- DOE Contract Number:
- AS02-76EV02364
- OSTI ID:
- 5208898
- Report Number(s):
- DOE/EV/02364-20; COO-2364-20; ON: DE82016232
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mechanisms for radiation damage in DNA. Progress report, January 1, 1981-December 31, 1981
Electron spin resonance and intermediate neglect of differential overlap molecular orbital study of the. pi. cations of (hydroxymethyl)uracil and (hydroxymethyl)cytosine. Evidence for internal hydrogen bonding
Electron spin resonance study of radicals produced by one-electron loss from 6-azauracil, 6-azathymine, and 6-azacytosine. Evidence for both sigma and. pi. radicals
Technical Report
·
Tue Sep 01 00:00:00 EDT 1981
·
OSTI ID:6314983
Electron spin resonance and intermediate neglect of differential overlap molecular orbital study of the. pi. cations of (hydroxymethyl)uracil and (hydroxymethyl)cytosine. Evidence for internal hydrogen bonding
Journal Article
·
Wed Feb 16 23:00:00 EST 1983
· J. Phys. Chem.; (United States)
·
OSTI ID:5903183
Electron spin resonance study of radicals produced by one-electron loss from 6-azauracil, 6-azathymine, and 6-azacytosine. Evidence for both sigma and. pi. radicals
Journal Article
·
Thu May 13 00:00:00 EDT 1982
· J. Phys. Chem.; (United States)
·
OSTI ID:6577121
Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
AMINES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL KILLING
CELL MEMBRANES
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CYTOSINE
DNA
ELECTRON SPIN RESONANCE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LIPIDS
MAGNETIC RESONANCE
MEMBRANES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADICALS
RESONANCE
THYMINE
URACILS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
AMINES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL KILLING
CELL MEMBRANES
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CYTOSINE
DNA
ELECTRON SPIN RESONANCE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LIPIDS
MAGNETIC RESONANCE
MEMBRANES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADICALS
RESONANCE
THYMINE
URACILS