Comparative study of the radioprotective effects of cysteamine, WR-2721, and WR-1065 in cultured human cells. [Reproductive death of cells following. gamma. irradiation used as indicator]

Journal Article · Thu Feb 01 00:00:00 EST 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575142· OSTI ID:6312192

Purdie, J W

The compounds, 2-mercaptoethylamine (cysteamine), S-2-(3-aminopropylamino)ethylphosphorothioic acid (WR-2721), and N-(2-mercaptoethyl)-1,3-diaminopropane (WR-1065) were tested for toxicity and radioprotective effect in cultured human cells using viability (reproductive death) as an indicator. WR-2721 and WR-1065 had no harmful effect at concentrations of 4 and 10 mM for periods up to 3 hr whereas cysteamine was toxic if left in contact with the cells for more than 30 min. The response of the cells to radiation was measured after a 30-min treatment with 4 mM of each protective agent. Survival curves were constructed and the following dose reduction factors (DRFs) were calculated: WR-2721, 1.3; cysteamine, 2.3; WR-1065, 2.9. Increasing the concentration of protective agent did not increase the DRF under these conditions except in the case of cysteamine. Extending the period of treatment with drug before irradiation increased the DRF for WR-2721 and WR-1065; values up to 1.8 and 3.4 were obtained with 10 mM WR-2721 and WR-1065, respectively. With these two compounds, the rate of development of protection with time is almost independent of concentration until the maximum is attained; it is probably controlled by the rate of transport across the cell membrane, in the case of WR-1065, and by the rate of hydrolysis of WR-2721.

• Research Organization: Defence Research Establishment, Ottawa, Canada
• OSTI ID: 6312192
• Journal Information: Radiat. Res.; (United States), Vol. 77:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
RADIOPROTECTIVE SUBSTANCES
AMINES
ANIMAL CELLS
SURVIVAL TIME
MEA
RADIOSENSITIVITY EFFECTS
TOXICITY
COMPARATIVE EVALUATIONS
BIOLOGICAL RADIATION EFFECTS
THIOLS
THIOPHOSPHORIC ACID ESTERS
CELL CULTURES
COBALT 60
EXPERIMENTAL DATA
GAMMA RADIATION
IRRADIATION
ISOLATED VALUES
MAN
TIME DEPENDENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COBALT ISOTOPES
DATA
DATA FORMS
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
HYDROCARBONS
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)