Autoradiographic determination of brain pH following middle cerebral artery occlusion in the rat
Journal Article
·
· Stroke; (United States)
A method of quantitative autoradiography using /sup 14/C-labelled 5,5-dimethyl-2, 4-oxazolidinedione (/sup 14/C-DMO) to evaluate the local changes in brain pH after ischemia is described. In normal control rats the calculated tissue pH values in gray matter were slightly lower than those in white matter, and there was no significant difference in the calculated pH among the various structures in cortical and subcortical gray matter. Four hours after a left middle cerebral artery (MCA) occlusion, marked reductions in /sup 14/C-DMO concentrations were demonstrated in the anterior two-thirds of the cerebral cortex and in the lateral part of the caudate nucleus indicating tissue acidosis in these areas. Although several assumptions are required for the calculation of pH in brain tissue, this method would appear very useful in the investigation of the altered metabolic state in ischemic brain.
- Research Organization:
- Montreal Neurological Inst., Quebec, Canada
- OSTI ID:
- 6306826
- Journal Information:
- Stroke; (United States), Journal Name: Stroke; (United States) Vol. 15:3; ISSN SJCCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550602 -- Medicine-- External Radiation in Diagnostics-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ARTERIES
AUTORADIOGRAPHY
AZOLES
BIOMEDICAL RADIOGRAPHY
BLOOD VESSELS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
DIAGNOSTIC TECHNIQUES
DISEASES
HETEROCYCLIC COMPOUNDS
ISCHEMIA
LABELLED COMPOUNDS
MAMMALS
MEDICINE
NERVOUS SYSTEM
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXAZOLES
PH VALUE
RADIOLOGY
RATS
RODENTS
VASCULAR DISEASES
VERTEBRATES
550602 -- Medicine-- External Radiation in Diagnostics-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ARTERIES
AUTORADIOGRAPHY
AZOLES
BIOMEDICAL RADIOGRAPHY
BLOOD VESSELS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
DIAGNOSTIC TECHNIQUES
DISEASES
HETEROCYCLIC COMPOUNDS
ISCHEMIA
LABELLED COMPOUNDS
MAMMALS
MEDICINE
NERVOUS SYSTEM
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXAZOLES
PH VALUE
RADIOLOGY
RATS
RODENTS
VASCULAR DISEASES
VERTEBRATES