Regional cerebral blood flow during hypoxia-ischemia in immature rats

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Stroke; (United States)

DOI:https://doi.org/10.1161/01.STR.19.2.245· OSTI ID:5234231

Vannucci, R C; Lyons, D T; Vasta, F

Immature rats subjected to a combination of unilateral common carotid artery ligation and hypoxia sustain brain damage confined largely to the ipsilateral cerebral hemisphere. To ascertain the extent and distribution of ischemic alterations in the brains of these small animals, we modified the Sakurada technique to measure regional cerebral blood flow using carbon-14 autoradiography. Seven-day-old rats underwent right common carotid artery ligation following which they were rendered hypoxic with 8% O2 at 37 degrees C. Before and during hypoxia, the rat pups received an injection of iodo(/sup 14/C)antipyrine for determination of regional cerebral blood flow. Blood flows to individual structures of the ipsilateral cerebral hemisphere were not influenced by arterial occlusion alone; flows to the contralateral hemisphere and to the brainstem and cerebellum actually increased by 25-50%. Hypoxia-ischemia was associated with decreases in regional cerebral blood flow of the ipsilateral hemisphere such that by 2 hours, flows to subcortical white matter, neocortex, striatum, and thalamus were 15, 17, 34, and 41% of control, respectively. The hierarchy of the blood flow reductions correlated closely with the distribution and extent of ischemic neuronal necrosis. However, unlike the pathologic pattern of this model, the degree of ischemia appeared homogeneous within each brain region. Blood flows to contralateral cerebral hemispheric structures were relatively unchanged from prehypoxic values, whereas flows to the brainstem and cerebellum nearly doubled and tripled, respectively. Thus, ischemia is the predominant factor that determines the topography of tissue injury to major regions of immature rat brain, whereas metabolic factors may influence the heterogeneous pattern of damage seen within individual structures.

• Research Organization: Pennsylvania State Univ. College of Medicine, Hershey (USA)
• OSTI ID: 5234231
• Journal Information: Stroke; (United States), Journal Name: Stroke; (United States) Vol. 19:2; ISSN SJCCA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
ANTIPYRETICS
ANTIPYRINE
AUTORADIOGRAPHY
AZOLES
BLOOD FLOW
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
ISCHEMIA
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PYRAZOLES
PYRAZOLINES
RATS
RODENTS
VASCULAR DISEASES
VERTEBRATES