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Unscheduled DNA synthesis in human bronchial epithelium treated with various chemical carcinogens in vitro

Journal Article · · J. Natl. Cancer Inst.; (United States)
OSTI ID:6304773
; ; ;
A system was developed in which organ culture of human bronchial epithelium was used in combination with autoradiography for quantitative measurement of unscheduled DNA synthesis (UDS) in bronchial epithelial cells. Human bronchi obtained at surgery were cut into small sections and treated with various carcinogens plus (methyl-/sup 3/H)thymidine in short-term organ culture. Significant numbers of silver grains, indicating UDS, were detected on the nuclei of epithelial cells of human bronchi treated with carcinogens, and the numbers were proportional to the concentrations of carcinogens. In this system seven representative carcinogens induced UDS. Four active metabolites of benzo(a)pyrene, and benz(a)anthracene also were found to induce very active UDS in human bronchial epithelium. These findings suggest that human bronchial epithelial cells can repair different types of DNA modification induced by chemical carcinogens.
Research Organization:
Japanese Foundation for Cancer Research, Tokyo
OSTI ID:
6304773
Journal Information:
J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 73:1; ISSN JNCIA
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
AROMATICS
AUTORADIOGRAPHY
AZINES
BENZANTHRACENE
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
BRONCHI
CARCINOGENS
CONDENSED AROMATICS
DNA REPLICATION
EPITHELIUM
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
LABELLED COMPOUNDS
METABOLITES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RESPIRATORY SYSTEM
RIBOSIDES
THYMIDINE
TISSUE CULTURES
TISSUES
TRITIUM COMPOUNDS