Chemical carcinogen-induced decreases in genomic 5-methyldeoxycytidine content of normal human bronchial epithelial cells
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
The genomic content of DNA 5-methyldeoxycytidine (m/sup 5/dC) was measured in dividing normal human bronchial epithelial cells treated with a broad range of chemical carcinogens. At noncytotoxic concentrations, all of the carcinogenic agents tested significantly reduced cellular DNA m/sup 5/dC content whereas the weakly carcinogenic and noncarcinogenic agents, benzo(e)pyrene and phenanthrene (respectively), did not. These reductions varied from 8% to 31% depending on the agent and the donor cells. The reduction is genomic m/sup 5/dC levels were concentration dependent for the carcinogenic polycyclic aromatic hydrocarbon benzo(a)pyrene. The authors speculate that carcinogen-induced perturbation of DNA m/sup 5/dC patterns may lead to heritable changes in gene expression and contribute to the molecular alterations involved in the initiation and the subsequent steps of the carcinogenesis process.
- Research Organization:
- National Institutes of Health, Bethesda, MD
- OSTI ID:
- 5503936
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:10; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANIMAL TISSUES
ANTIGENS
AROMATICS
ATP
AZINES
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
BRONCHI
CARCINOGENS
CHEMICAL REACTIONS
CONDENSED AROMATICS
CYTIDINE
DAYS LIVING RADIOISOTOPES
DIMETHYLBENZANTHRACENE
DNA
EPITHELIUM
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
METHYLATION
NITROSO COMPOUNDS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENANTHRENE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PYRIMIDINES
RADIOISOTOPES
RESPIRATORY SYSTEM
RIBOSIDES
TISSUES
TOXIC MATERIALS
TOXINS
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANIMAL TISSUES
ANTIGENS
AROMATICS
ATP
AZINES
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
BRONCHI
CARCINOGENS
CHEMICAL REACTIONS
CONDENSED AROMATICS
CYTIDINE
DAYS LIVING RADIOISOTOPES
DIMETHYLBENZANTHRACENE
DNA
EPITHELIUM
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
METHYLATION
NITROSO COMPOUNDS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENANTHRENE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PYRIMIDINES
RADIOISOTOPES
RESPIRATORY SYSTEM
RIBOSIDES
TISSUES
TOXIC MATERIALS
TOXINS