Detection of aryl hydrocarbon hydroxylase activity in normal and neoplastic human breast epithelium

Journal Article · Mon Jan 28 04:00:00 EST 1980 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(80)90343-4· OSTI ID:5413865

Greiner, J W; Malan-Shibley, L B; Janss, D H

Studies were conducted to determine whether normal and/or neoplastic (MCF-7) human breast epithelial cells contain the microsomal aryl hydrocarbon hydroxylase (AHH) which catalyses the conversion of polycyclic aromatic hydrocarbons (PAH) to carcinogenic intermediates. Low constitutive levels of AHH activity were found in homogenates of both normal human breast epithelial and MCF-7 cells. The addition of 7,12-dimethylbenz(a)anthracene (DMBA) to the culture medium of either cell type significantly increased AHH activity. Peak induction of hydroxylase activity occurred following the in vitro addition of 10 ..mu..M DMBA. A time course of DMBA-induced AHH activity in both normal human breast epithelium and MCF-7 cells revealed maximal induction 16 hr after 10 ..mu..M DMBA was added to the culture medium. Benzo(a)pyrene (BP), 3-methylcholanthrene (MCA) and benz(a)anthracene (BA) also induced AHH activity in normal and MCF-7 cells. For example, the addition of 10 ..mu..M BP to the culture medium of either normal human breast epithelial or MCF-7 cells for 16 hr increased AHH activity 13.8 and 65.3-fold, respectively. For all PAH, the magnitude of AHH induction was substantially greater in MCF-7 than normal breast epithelial cells. Finally, ..cap alpha..-naphthoflavone inhibited BA-induced AHH activity in MCF-7 cells. The study demonstrates the presence of a PAH-inducible AHH enzyme(s) in normal human breast epithelial cells grown in primary culture and in the human breast tumor cell line, MCF-7.

• Research Organization: Frederick Cancer Research Center, MD
• OSTI ID: 5413865
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 26:4; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
550200 -- Biochemistry
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZANTHRACENE
BENZOPYRENE
BODY
CARCINOMAS
CELL CULTURES
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
DISEASES
ENZYME ACTIVITY
ENZYMES
EPITHELIUM
GLANDS
HYDROCARBONS
HYDROXYLASE
MAMMALS
MAMMARY GLANDS
MAN
METABOLIC ACTIVATION
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
TISSUES
TUMOR CELLS
VERTEBRATES