Genetic manipulation of ligninolytic streptomyces and generation of improved lignin-to-chemical bioconversion strains
Streptomyces viridosporus T7A, when used in solid-state fermentation, degrades lignin at high yields to a water-soluble modified polymer, acid precipitable polymeric lignin (APPL) that is useful as an antioxidant, surfactant, and potentially as a component of adhesives and resins. Enhanced strains generated from ultraviolet irradiation mutagenesis and protoplast fusion produced up to 90% more APPL from corn stover lignocellulose than did the wildtype, and they were stable and produced APPL at a faster rate and to a higher final yield than did parental strain T7A. APPLs produced by the wildtype and selected mutants were chemicaly similar polyphenols, but some catabolic enzymes of the genetically manipulated strains were produced in greater amounts.
- Research Organization:
- Univ. of Idaho, Moscow
- OSTI ID:
- 6299673
- Report Number(s):
- CONF-840509-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 16:14; ISSN BIBSB
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ADHESIVES
AGRICULTURAL WASTES
ANTIOXIDANTS
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CEREALS
CHEMICAL FEEDSTOCKS
DELIGNIFICATION
ENZYMES
FERMENTATION
GENETIC ENGINEERING
GRASS
LIGNIN
MAIZE
MICROORGANISMS
MUTAGENESIS
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
POLYMERS
POLYSACCHARIDES
PRODUCTIVITY
SACCHARIDES
STREPTOMYCES
SURFACTANTS
SYNTHESIS
WASTES