Enzymology of lignocellulose bioconversion by Streptomyces viridosporus
Thesis/Dissertation
·
OSTI ID:6896660
Significant progress has been made in lignin biodegradation research since 1983, when lignin peroxidases were discovered in fungi. A similar breakthrough in bacterial lignin biodegradation research is anticipated. Several laboratories have successfully demonstrated the ability of bacteria to mineralize ({sup 14}C)-lignin lignocelluloses as well as {sup 14}C-labelled synthetic lignins. Attempts are being made to identify the key enzymes involved. In this dissertation, two studies are presented which address the enzymology of lignin biodegradation by Streptomyces viridosporus. The first study compares selected extracellular enzyme of wild-type and genetically manipulated strains with enhanced abilities to produced a water soluble lignin degradation intermediate, designated acid-precipitable polymeric lignin (APPL). UV irradiation mutant T7A-81 and protoplast fusion recombinant SR-10 had higher and longer persisting peroxidase, esterase, and endoglucanase activity than did the wild type strain T7A. An extracellular lignocellulose-induced peroxidase with some similarities to fungal ligninases was described for the first time in Streptomyces. The second study describes purification and characterization of an extracellular lignin peroxidase produced by S. viridosporus T7A. This is the first report of a lignin peroxidase in any bacterium.
- Research Organization:
- Idaho Univ., Moscow, ID (USA)
- OSTI ID:
- 6896660
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
560130 -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIODEGRADATION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELLULASE
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESTERASES
FRACTIONATION
GLYCOSYL HYDROLASES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGNIN
MICROORGANISMS
MUTANTS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
POLYSACCHARIDES
RADIATION INDUCED MUTANTS
RADIATIONS
SACCHARIDES
SEPARATION PROCESSES
STREPTOMYCES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
560130 -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIODEGRADATION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELLULASE
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESTERASES
FRACTIONATION
GLYCOSYL HYDROLASES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGNIN
MICROORGANISMS
MUTANTS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
POLYSACCHARIDES
RADIATION INDUCED MUTANTS
RADIATIONS
SACCHARIDES
SEPARATION PROCESSES
STREPTOMYCES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION