Comparison of extracellular peroxidase- and esterase-deficient mutants of Streptomyces viridosporus T7A
- Univ. of Idaho, Moscow (United States)
Peroxidase-deficient mutants of the lignin-degrading bacterium Streptomyces viridosporus T7A were screened for their production of acid-precipitable polymeric lignin, extracellular peroxidases and esterases, and immunoreactivities against a polyclonal antibody produced against electrophoretically purified peroxidase isoform P3 of wild-type S. viridosporus. The mutants showed diminished abilities to solubilize lignin and produce acid-precipitable polymeric lignin. Their peroxidase activities were decreased, and their esterase production patterns were altered. Western immunoblots demonstrated that the mutants produced proteins immunologically reactive with the antibody, but with different mobilities from those of wild-type proteins. These findings confirm a direct role for peroxidases in lignin solubilization. They also indicate a possible role for esterases.
- DOE Contract Number:
- FG07-86ER13586
- OSTI ID:
- 7206405
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 58:3; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
ESTERASES
ENZYME ACTIVITY
LIGNIN
BIODEGRADATION
PEROXIDASES
COMPARATIVE EVALUATIONS
IMMUNE REACTIONS
MUTANTS
STREPTOMYCES
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
EVALUATION
HYDROLASES
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
POLYSACCHARIDES
PROTEINS
SACCHARIDES
550200* - Biochemistry
090900 - Biomass Fuels- Processing- (1990-)