Comparison of extracellular peroxidase- and esterase-deficient mutants of Streptomyces viridosporus T7A
- Univ. of Idaho, Moscow (United States)
Peroxidase-deficient mutants of the lignin-degrading bacterium Streptomyces viridosporus T7A were screened for their production of acid-precipitable polymeric lignin, extracellular peroxidases and esterases, and immunoreactivities against a polyclonal antibody produced against electrophoretically purified peroxidase isoform P3 of wild-type S. viridosporus. The mutants showed diminished abilities to solubilize lignin and produce acid-precipitable polymeric lignin. Their peroxidase activities were decreased, and their esterase production patterns were altered. Western immunoblots demonstrated that the mutants produced proteins immunologically reactive with the antibody, but with different mobilities from those of wild-type proteins. These findings confirm a direct role for peroxidases in lignin solubilization. They also indicate a possible role for esterases.
- DOE Contract Number:
- FG07-86ER13586
- OSTI ID:
- 7206405
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:3; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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090900 -- Biomass Fuels-- Processing-- (1990-)
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59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIODEGRADATION
CARBOHYDRATES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
ESTERASES
EVALUATION
HYDROLASES
IMMUNE REACTIONS
LIGNIN
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
POLYSACCHARIDES
PROTEINS
SACCHARIDES
STREPTOMYCES