Catabolic fate of Streptomyces viridosporus T7A-Produced, acid precipitable polymeric lignin upon incubation with ligninolytic Streptomyces species and Phanerochaete chrysosporium
Degradation of ground and hot-water-extracted corn stover (Zea mays) lignocellulose by Streptomyces viridosporus T7A generates a water-soluble lignin degradation intermediate termed acid-precipitable polymeric lignin (APPL). The further catabolism of T7A-APPL by S. viridosporus T7A, S. badius 252, and S. setonii75Vi2 was followed for 3 weeks. APPL catabolism by Phanerochaete chrysosporium was followed in stationary cultures in a low-nitrogen medium containing 1% (wt/vol) glucose and 0.05% (wt/vol) T7A-APPL. Metabolism of the APPL was followed by turbidometric assay (600 nm) and by direct measurement of APPL recoverable from the medium. Accumulation and disappearance of soluble low-molecular-weight products of APPL catabolism were followed by gas-liquid chromatography and by high-pressure liquid chromatography, utilizing a diode array detector. Mineralization of a (/sup 14/C-lignin)APPL was also followed. The percent /sup 14/C recovered as /sup 14/CO/sub 2/, /sup 14/C-APPL, /sup 14/C-labeled water-soluble products, and cell mass-associated radioactivity, were determined for each microorganism after 1 and 3 weeks of incubation in bubbler tube cultures at 37/sup 0/C. P. chrysosporium evolved the most /sup 14/CO/sub 2/, and S. viridosporus gave the greatest decrease in recoverable /sup 14/C-APPL. The results show that S. badius was not able to significantly degrade the APPL, while the other microorganisms demonstrated various APPL-degrading abilities.
- Research Organization:
- Univ. of Idaho, Moscow
- DOE Contract Number:
- AS07-80ER10775
- OSTI ID:
- 5095423
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 51:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FUNGI
METABOLISM
LIGNIN
BIODEGRADATION
STREPTOMYCES
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
TRACER TECHNIQUES
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
550501* - Metabolism- Tracer Techniques