Production and characterization of polymeric lignin degradation intermediates from two different Streptomyces spp
Previous investigations have identified a quantitatively major intermediate of lignin degradation by Streptomyces viridosporus. The intermediate, a modified lignin polymer, acid-precipitable polymeric lignin (APPL), is released as a water-soluble catabolite and has been recovered in amounts equivalent to 30% of the lignin originally present in a corn stover lignocellulose substrate after degradation by this actinomycete. In the present work, APPLs were collected at various time intervals from cultures of two highly ligninolytic Streptomyces sp. strains, S. viridosporus T7A and S. badius 252, growing on corn stover lignocellulose. APPL production was measured over time, and the chemistry of APPLs produced by each organism after different time intervals was compared. Chemical characterizations included assays for lignin, carbohydrate, and ash contents, molecular weight distributions by gel permeation chromatography, and chemical degradation analyses by permanganate oxidation, acidolysis, and alkaline ester hydrolysis. Differences between the organisms were observed in the cultural conditions required for APPL production and in the time courses of APPL accumulation. S. viridosporus produced APPL in solid-state fermentation over a 6- to 8-week incubation period, whereas S. badius produced as much or more APPL, but only in liquid culture and over a 7- to 8-day incubation period. The chemistry of the APPLs produced also differed. S. viridosporus APPL was more lignin-like than that of S. badius and was slowly modified further over time, although no change in molecular weight distribution over time was observed. In contrast, S. badius APPL was less lignin-like and increased substantially in average molecular weight over time.
- Research Organization:
- Univ. of Idaho, Moscow
- DOE Contract Number:
- AS07-80ER10775
- OSTI ID:
- 5736833
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 49:2; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
ASHES
BACTERIA
BIOCONVERSION
BIODEGRADATION
BIOSYNTHESIS
CARBOHYDRATES
CEREALS
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DECOMPOSITION
FERMENTATION
GEL PERMEATION CHROMATOGRAPHY
GRASS
GROWTH
LIGNIN
MAIZE
MICROORGANISMS
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
POLYSACCHARIDES
RESIDUES
SACCHARIDES
SEPARATION PROCESSES
SPECIES DIVERSITY
STREPTOMYCES
SYNTHESIS
WASTES