Effect of activated lymphocytes on the regulation of hematopoiesis: suppression of in vitro granulopoiesis and erythropoiesis by OKT8+ Ia- T cells induced by concanavalin-A stimulation

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · Exp. Hematol.; (United States)

OSTI ID:6299592

Harada, M; Odaka, K; Kondo, K; Nakao, S; Ueda, M; Matsue, K; Mori, T; Matsuda, T

The effects of activated lymphocytes were studied in the regulation of in vitro hematopoiesis. Peripheral blood lymphocytes stimulated by concanavalin A (Con A) were cocultured with normal bone marrow cells in the assay system of hematopoietic stem cells. Con-A-stimulated lymphocytes and their supernatants showed significant suppression of in vitro growth of myeloid and erythroid progenitor cells (CFU-C, CFU-E, and BFU-E). Suppressive activity detected in the T-cell fraction was completely abolished by treatment with OKT3 or OKT8 monoclonal antibody and complement and 20 Gy radiation but not OKT4 or OKIa1 antibody and complement. These observations indicate that peripheral blood lymphocytes can be induced by Con-A stimulation to become suppressor T cells for myeloid and erythroid progenitor cells that are OKT8 positive, Ia negative, and radiosensitive. Together with our previous observation that CFU-C suppressor cells induced by alloantigen stimulation are radioresistant and OKT8- and Ia-positive T cells, it is suggested that in vitro hematopoiesis may be regulated by heterogeneous subpopulations of activated T-lymphocytes.

• Research Organization: Kanazawa Univ. School of Medicine, Japan
• OSTI ID: 6299592
• Journal Information: Exp. Hematol.; (United States), Vol. 9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
IMMUNE REACTIONS
RADIOSENSITIVITY
ANTIGEN-ANTIBODY REACTIONS
BLOOD FORMATION
BONE MARROW CELLS
CELL CULTURES
COMPLEMENT
CONCANAVALIN
ERYTHROCYTES
ERYTHROPOIESIS
HEMATOPOIETIC SYSTEM
IRRADIATION
MONOCLONAL ANTIBODIES
STEM CELLS
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
HEMAGGLUTININS
LEUKOCYTES
MATERIALS
SOMATIC CELLS
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