Liquid chromatographic determination of benzo(a)pyrene in total particulate matter of cigarette smoke

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · J. Assoc. Off. Anal. Chem.; (United States)

OSTI ID:6295365

Tomkins, B A; Jenkins, R A; Griest, W H; Reagan, R R; Holladay, S K

The benzo(a)pyrene (BaP) delivery of reference and commercially available tobacco cigarettes, as well as reference and placebo marijuana cigarettes, is determined using a sequential liquid chromatographic/liquid chromatographic procedure. The total particulate matter of sample cigarette smoke is collected using a Cambridge filter pad, which is ultrasonically extracted with acetone. The resulting extract is filtered, then fractionated using semipreparative-scale normal phase liquid chromatography (LC). Quantitative determination is achieved using analytical-scale reverse phase LC equipped with a fluorescence detector. The method is precise (+/- 10-15% relative standard deviation) and yields 85% or better BaP recovery at the ng/cig. level. A single pad may be analyzed in 8 person-hours, while a more typical lot of 12 pads (6 pads each for 2 cigarette brands) may be analyzed in 10 person-days.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 6295365
• Journal Information: J. Assoc. Off. Anal. Chem.; (United States), Journal Name: J. Assoc. Off. Anal. Chem.; (United States) Vol. 5; ISSN JANCA
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560306 -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AROMATICS
BENZOPYRENE
CHROMATOGRAPHY
COLLOIDS
CONDENSED AROMATICS
DISPERSIONS
FLUORESCENCE
FRACTIONATION
HERBS
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
MARIHUANA
ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
PLANTS
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
TOBACCO SMOKES