A high-performance liquid chromatographic determination of major phenolic compounds in tobacco smoke

Journal Article · Tue May 01 04:00:00 EDT 1990 · Journal of Chromatographic Science; (USA)

DOI:https://doi.org/10.1093/chromsci/28.5.239· OSTI ID:6060960

Risner, C H; Cash, S L ^[1]

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1. R. J. Reynolds Tobacco Company, Winston-Salem, NC (USA)

A high-performance liquid chromatographic (HPLC) method is developed that simultaneously quantifies the dihydroxy compounds hydroquinone, resorcinol, and catechol and the monohydroxy compounds phenol, m + p-cresol and o-cresol in cigarette smoke. Particulate matter samples collected on Cambridge pads and in impingers by conventional trapping techniques are simply (no derivatization required) subjected to reversed-phase gradient liquid chromatography. Samples of both mainstream and sidestream smoke can be analyzed. Selective fluorescence detection is used to monitor the mobile phase effluent, by which these phenolic compounds are detected in the nanogram range. The detector response is linear, overall precision is good, and recoveries are greater than 95 percent. The total run time, excluding extraction, is one hour. The procedure has been applied to tobacco products whose smoke contains varying amounts of these phenols. Kentucky Reference Cigarette 1R4F was found to contain substantially more of these compounds than a new cigarette that heats but does not burn tobacco (New Cigarette). The method is compared with other procedures used to determine phenolics in cigarette smoke.

• OSTI ID: 6060960
• Journal Information: Journal of Chromatographic Science; (USA), Journal Name: Journal of Chromatographic Science; (USA) Vol. 28:5; ISSN JCHSB; ISSN 0021-9665
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540120* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
AEROSOLS
AIR POLLUTION
AROMATICS
CHROMATOGRAPHY
COLLOIDS
DISPERSIONS
FLUORESCENCE
HYDROXY COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
ORGANIC COMPOUNDS
PHENOLS
POLLUTION
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
TOBACCO SMOKES