Liquid chromatographic determination of benzo(a)pyrene in natural, synthetic, and refined crudes

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00231a019· OSTI ID:6396201

Tomkins, B A; Reagan, R R; Caton, J E; Griest, W H

Benzo(a)pyrene (BaP) is isolated and quantitated by using a sequential high-pressure liquid chromatographic (HPLC) procedure. The sample is first injected onto a semipreparative HPLC column containing a bonded polar aminocyano (PAC) packing material, from which a BaP-enriched fraction is obtained. This isolate is then reinjected onto a Zorbax ODS reversed-phase analytical-scale column, and fluorescence detection is used to quantitate BaP. This procedure is applicable to samples with BaP concentrations ranging from 0.02 to 500 ..mu..g/g. The precision is nominally +- 6% (relative standard deviation), and the accuracy compares favorably with that displayed by more tedious methods. Recoveries, as determined by counting a radioactive BaP tracer, usually exceed 95%. Two samples may be processed per person-day.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6396201
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 53:8; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
010600 -- Coal
Lignite
& Peat-- Properties & Composition
02 PETROLEUM
023000 -- Petroleum-- Properties & Composition
04 OIL SHALES AND TAR SANDS
040500 -- Oil Shales & Tar Sands-- Properties & Composition
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104 -- Spectral Procedures-- (-1987)
400105* -- Separation Procedures
ACCURACY
AROMATICS
BENZOPYRENE
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COAL LIQUIDS
COLUMN PACKING
CONDENSED AROMATICS
EMISSION SPECTROSCOPY
ENERGY SOURCES
FLUIDS
FLUORESCENCE SPECTROSCOPY
FOSSIL FUELS
FRACTIONATION
FUELS
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
LIQUIDS
MINERAL OILS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PACKINGS
PETROLEUM
QUANTITATIVE CHEMICAL ANALYSIS
SENSITIVITY
SEPARATION PROCESSES
SHALE OIL
SPECTROSCOPY
SYNTHETIC FUELS
TAR