Arginine vasopressin promotes growth of rat glomerular mesangial cells in culture

Journal Article · Tue Nov 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6291514

Ganz, M B; Pekar, S K; Perfetto, M C; Sterzel, R B ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (USA)

Arginine vasopressin (AVP) binds specifically to vascular smooth muscle-like mesangial cells (MCs) and affects contraction. The authors tested whether this peptide also modulates growth behavior of rat MCs in early subculture (passage 2-5). Subconfluent, serum-starved MCs were exposed to AVP in the presence or absence of insulin. To assess DNA replication, MC uptake of ({sup 3}H)thymidine was determined on days 1, 2, and 3. AVP alone averaged a 1.97-fold increase in DNA synthesis at 24 h, whereas the mean stimulatory effects of AVP at 48 and 72 h were 7.21- and 5.42-fold, respectively. MCs exposed simultaneously to AVP and insulin showed potentiation of the mitogenic response to AVP alone. The V{sub 1}-receptor antagonist (1-({beta}-mercapto-{beta},{beta}-cyclopentamethylene proprionic acid), 2-(O-methyl-Tyr)-Arg)vasopressin (PMP) inhibited only AVP-induced promotion of MC growth. The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) acutely stimulated MC proliferation but did not add to the AVP effect. They conclude that (1) AVP is strongly and specifically mitogenic for rat MCs, (2) the proliferative effect of AVP is mediated by TPA-like activation of protein kinase C, and (3) a maximal rise of Ca{sub i} need not be essential for the induction of AVP-elicited mitogenesis of MCs.

• OSTI ID: 6291514
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ARGININE
BIOCHEMISTRY
CARBOXYLIC ACIDS
CELL CULTURES
CHEMISTRY
DNA REPLICATION
ENZYMES
HORMONES
HYDROGEN COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MUSCLES
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY HORMONES
PROTEINS
RATS
RECEPTORS
RODENTS
TRANSFERASES
TRITIUM COMPOUNDS
UPTAKE
VASOPRESSIN
VERTEBRATES