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Vasopressin receptors from cultured mesangial cells resemble V/sub 1a/ type

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5982914
; ; ;

Mesangial cells respond to vasopressin by contraction and increased prostaglandin production. The purpose of the present study is to characterize vasopressin receptors from these cells. Glomeruli were isolated from rat kidneys and plated for explant growth of mesangial cells. Membranes were prepared from cells grown for 6 wk and tested for their ability to bind (/sup 3/H)vasopressin (lysine vasopressin). These membranes contained a single class of specific vasopressin binding sites. Vasopressin induced a dose-dependent accumulation of labeled inositol phosphates in myo(/sup 3/H)inositol-prelabeled mesangial cells incubated in the presence of 10 mM of Li. Conversely, vasopressin failed to alter the adenylate cyclase activity of mesangial cell membranes. Competition experiments with a series of vasopressin structural analogues that have different degrees affinity for V/sub 2/- (renal), V/sub 1a/- (vascular and hepatic), and V/sub 1b/- (adenohypohyseal) receptors, indicated that vasopressin receptors from rat glomerular mesangial cells resemble the V/sub 1a/-receptor subtype.

Research Organization:
Centre National de la Recherche Scientifique, Montpellier (France)
OSTI ID:
5982914
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:1; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
GLOMERULI
HORMONES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
MORPHOLOGY
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TRITIUM COMPOUNDS
UPTAKE
VASOPRESSIN
VERTEBRATES