AVP-induced Ca fluxes and contraction of rat glomerular mesangial cells
- Univ. of Colorado School of Medicine, Denver (USA)
Arginine vasopressin (AVP) is known to exert Ca mobilization and contraction in glomerular mesangial cells and vascular smooth muscle cells. However, the relationship between changes in intracellular Ca and transmembrane Ca fluxes is not clear at the present time. Therefore, this study was undertaken to determine the effect of AVP on cytosolic calcium ((Ca{sup 2+}){sub i}) and Ca fluxes as estimated by measurements of {sup 45}Ca{sup 2+} efflux. Changes of (Ca{sup 2+}){sub i} in response to AVP were directly measured in monolayers of adherent cultured mesangial cells. AVP induced rapid concentration-dependent increases in (Ca{sup 2+}){sub i} and Ca{sup 2+} efflux. AVP also induced contraction of mesangial cells. This effect was blocked only by the V{sub 1} (pressor)-antagonist, d(CH{sub 2}){sub 5}Tyr(Me)AVP. Stimulation of Ca{sup 2+} efflux and changes in (Ca{sup 2+}){sub i} by AVP completely desensitized the mesangial cells to a subsequent identical challenge of AVP with no cross-tachyphylaxis to other hormones. In Ca{sup 2+}-containing conditions, lanthanum ion-reduced AVP evoked (Ca{sup 2+}){sub i} stimulation to the value observed in Ca{sup 2+}-free medium. The Ca{sup 2+} channel blocker, verapamil, partially inhibited AVP-induced Ca{sup 2+} influx but totally blocked the increase in (Ca{sup 2+}){sub i} induced by high K. These results indicate that AVP rapidly increases (Ca{sup 2+}){sub i} in cultured rat mesangial cells in part by mobilizing Ca{sup 2+} from dantrolene-sensitive intracellular pools that are activated by receptor-operated Ca{sup 2+} channels; a secondary effect of AVP on voltage-dependent Ca{sup 2+} channels was also observed.
- OSTI ID:
- 7191952
- Journal Information:
- American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:1; ISSN 0002-9513; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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ACETIC ACID ESTERS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
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