Small unilamellar vesicles as reagents: a chemically defined, quantitative assay for lectins
Samll unilamellar vesicles containing synthetic glycolipids can be prepared. These vesicles are aggregated by the appropriate lectin (Orr et al., 1979; Rando and Bangerter, 1979; Slama and Rando, 1980). It is shown here that extent of aggregation of these vesicles as measured by light scattering at 360 nm, is, under certain conditions, linear with amount of lectin added. This forms the basis of a rapid and simple quantitative assay for lectins using the modified vesicles as a defined chemical substrate. The assay is sensitive to lectin concentrations in the low ..mu..g range. The assay is applied here to studies on concanavalin A, Ricinus communis agglutinin and the ..cap alpha..-fucosyl binding lectin from Ulex europaeus (Type I).
- Research Organization:
- Harvard Medical School, Boston, MA (USA). Dept. of Pharmacology
- DOE Contract Number:
- AC02-79EV10268
- OSTI ID:
- 6289752
- Report Number(s):
- DOE/EV/10268-2; ON: DE81030365
- Country of Publication:
- United States
- Language:
- English
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