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Ozone interaction with rodent lung: effect on sulfhydryls and sulfhydryl-containing enzyme activities

Journal Article · · J. Lab. Clin. Med.; (United States)
OSTI ID:6288862
; ; ;
Exposure of rats or rat lung homogenates to 2 ppM O/sub 3/ reduced total, protein, and non-protein sulfhydryl fractions in a dose-related fashion. The non-protein fraction was more susceptible to O/sub 3/ attack. Chronic O/sub 3/ exposure (0.8 ppM, 10 days) had no effect. Activities of SH-containing enzymes, glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD), in lung tissue decreased 10 to 15 and 30%, respectively, after 6 to 8 h exposure to 2 ppM O/sub 3/. In vitro exposure (2 to 3 ppM) led to reductions of 20 and 67%. Activities of cytochrome C reductases decreased 20% after acute exposure but increased 20% after chronic. Chronic exposure increased SH-generating enzyme, G6PD, activity in lung tissue 32% but had little effect on GR activity. Lipid peroxidation (as possible mechanism for SH oxidation) not affected by in vivo exposure but was increased almost 100% in vitro. G6PD activity signals hexose-monophosphate shunt pathway for NADPH production. This pathway is also active in various other diseases (e.g., toxic liver injury).
OSTI ID:
6288862
Journal Information:
J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 80; ISSN JLCMA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COENZYMES
CYTOCHROMES
DEHYDROGENASES
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
GLUCOSE
GLUTATHIONE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HOMOGENATES
IN VITRO
IN VIVO
KINETICS
LIPIDS
LUNGS
MAMMALS
MONOSACCHARIDES
NADP
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDATION
OXIDOREDUCTASES
OZONE
PEPTIDES
PEROXIDASES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
RODENTS
SACCHARIDES
THIOLS
VERTEBRATES