Studies on biochemical effects of nitrogen dioxide. II. Changes of the protective systems in rat lungs and of lipid peroxidation by acute exposure

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398209530150· OSTI ID:5095622

Sagai, M; Ichinose, T; Oda, H; Kubota, K

This work was done to clarify the relation between the change of lipid peroxidation and the protective systems in lungs after NO/sub 2/ exposure. JCL:Wistar 8-wk-old male rats were exposed continuously to 10 ppm NO/sub 2/ for 2 wk. Lipid peroxidation, measured by ethane exhalation in the breath of the rats and by the reaction of thiobarbituric acid with lung homogenates, increased to a maximum at 3 d after a decline at 1 d, and then returned to the initial level (of d 0). Activities of glutathione peroxidase (GPx), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), disulfide reductase (DSR), and superoxide dismutase (SOD) in the 105,000 x g supernatant of lung homogenates were depressed slightly at 1 d. Thereafter, they increased significantly to their maximum levels from 5 to 10 d, and these maximum levels were maintained until d 14. The pattern of change of these protective enzymes was symmetric to that of lipid peroxidation after 3 d. The order of the ratio of the increased value to the initial value was G6PD > DSR > 6PGD > GR > GPx > SOD. The time course of nonprotein sulfydryls was similar to that of the protective enzymes. In contrast, the amounts of vitamin E increased to a maximum at 2 d and then returned to the initial level. The periodic change of vitamin E was similar to that of lipid peroxidation rather than that of the protective enzymes. These results suggest that the ability of the enzyme systems in lungs to protect against NO/sub 2/ fluctuated in a complex manner and the activities of the protective enzymes varied inversely with lipid peroxidation.

• Research Organization: National Inst. for Environmental Studies, Tsukuba-Ibaraki, Japan
• OSTI ID: 5095622
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 9:1; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CHALCOGENIDES
ENZYME ACTIVITY
KINETICS
LIPIDS
LUNGS
MAMMALS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES