Modulation of macrophage-mediated cytotoxicity by kerosene soot: Possible role of reactive oxygen species

Journal Article · Sat May 01 04:00:00 EDT 1993 · Environmental Research; (United States)

DOI:https://doi.org/10.1006/enrs.1993.1067· OSTI ID:6247466

Arif, J M; Khan, S G; Ashquin, M; Rahman, Q ^[1]

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1. Industrial Toxicology Research Centre, Lucknow (India)

The involvement of reactive oxygen species (ROS) in the cytotoxicity of soot on rat alveolar macrophages has been postulated. A single intratracheal injection of soot (5 mg) in corn oil significantly induced the macrophage population, hydrogen peroxide (H[sub 2]O[sub 2]) generation, thiobarbituric acid (TBA)-reactive substanced of lipid peroxidation, and the activities of extracellular acid phosphatase (AP) and lactate dehydrogenase (LDH) at 1, 4, 8, and 16 days of postinoculation. The activities of glutathione peroxidase (GPX) and catalase (CAT) were significantly inhibited at all the stages, while glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) showed a different pattern. These results show that soot is cytotoxic to alveolar macrophages and suggest that ROS may play a primary role in the cytotoxic process. 28 refs., 4 figs., 1 tab.

• OSTI ID: 6247466
• Journal Information: Environmental Research; (United States), Journal Name: Environmental Research; (United States) Vol. 61:2; ISSN ENVRAL; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID PHOSPHATASE
ALDEHYDES
ANIMAL CELLS
ANTIOXIDANTS
BIOCHEMISTRY
BIOLOGICAL STRESS
BODY
CARBOHYDRATES
CATALASE
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DISTILLATES
DRUGS
ELEMENTS
ENERGY SOURCES
ENZYME ACTIVITY
ENZYMES
ESTERASES
FOSSIL FUELS
FUELS
GAS OILS
GLUCOSE
GLUTATHIONE
GROWTH
HEMIACETAL DEHYDROGENASES
HEXOSES
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROLASES
INHIBITION
KEROSENE
LACTATE DEHYDROGENASE
LIQUID FUELS
LUNGS
MACROPHAGES
MONOSACCHARIDES
NONMETALS
ORGANIC COMPOUNDS
ORGANS
OXIDIZERS
OXIDOREDUCTASES
OXYGEN
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDASES
PEROXIDES
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
PHAGOCYTES
PHAGOCYTOSIS
PHOSPHATASES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
SACCHARIDES
SOMATIC CELLS
SOOT
TOXICITY