Effects of short-term, single and combined exposure to low-level NO sub 2 and O sub 3 on lung tissue enzyme activities in rats

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287399009531392· OSTI ID:6919152

Lee, J S; Mustafa, M G; Afifi, A A ^[1]

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1. Univ. of California, Los Angeles (USA)

To examine the pulmonary effects of relatively low levels of NO{sub 2} and O{sub 3}, and test for any possible interaction in their effects, we exposed 3-mo-old male Sprague-Dawley rats, free of specific pathogens, to either filtered room air (control) or 1.20 ppm (2256 micrograms/m3) NO{sub 2}, 0.30 ppm (588 micrograms/m3) O{sub 3}, or a combination of the two oxidants continuously for 3 d. We studied a series of parameters in the lung, including lung weight, and enzyme activities related to NADPH generation, sulfhydryl metabolism, and cellular detoxification. The results showed that relative to control, exposure to NO2 caused small but nonsignificant changes in all the parameters; O{sub 3} caused significant increases in all the parameters except for superoxide dismutase; and a combination of NO{sub 2} and O{sub 3} caused increases in all the parameters, and the increases were greater than those caused by NO{sub 2} or O{sub 3} alone. Statistical analysis of the data showed that the effects of combined exposure were synergistic for 6-phosphogluconate dehydrogenase, isocitrate dehydrogenase, glutathione reductase, and superoxide dismutase activities, and additive for glutathione peroxidase and disulfide reductase activities, but indifferent from those of O{sub 3} exposure for other enzyme activities.

• OSTI ID: 6919152
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Journal Name: Journal of Toxicology and Environmental Health; (USA) Vol. 29:3; ISSN JTEHD; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CHALCOGENIDES
COENZYMES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
LUNGS
MAMMALS
MATHEMATICS
NADP
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OZONE
RADICALS
RATS
REGRESSION ANALYSIS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
STATISTICS
SULFHYDRYL RADICALS
SUPEROXIDE DISMUTASE
SYNERGISM
TOXICITY
VERTEBRATES