Detection of liposomes in portal blood following oral administration
125Iodine-labeled human immunoglobulin G-encapsulated dipalmitoyl phosphatidylcholine liposomes were prepared with or without asialoganglioside. Distribution of radioactivity following the oral administration of these liposomes in rats was checked in liver and in blood of the portal vein and heart. Gel filtration of plasma from the portal vein showed the presence of intact liposomes and protein. In contrast, neither liposomes nor protein were detected in cardiac blood but only lower molecular weight materials. The presence of liposomes in portal blood was further suggested from experiments using 6-carboxyfluorescein-entrapped liposomes. The level of liposomes in portal venous blood plasma was found to be lower in asialoganglioside liposomes. But this level could be increased substantially, almost to that in liposomes without asialoganglioside, by injection (iv) of asialofetuin.
- Research Organization:
- Indian Institute of Chemical Biology, Calcutta
- OSTI ID:
- 6287332
- Journal Information:
- J. Appl. Biochem.; (United States), Journal Name: J. Appl. Biochem.; (United States) Vol. 5-6; ISSN JABID
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DYES
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
FLUORESCEIN
GANGLIOSIDES
GLANDS
GLOBULINS
HEART
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIPIDS
LIPOSOMES
LIVER
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORAL ADMINISTRATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHENOLS
PHOSPHOLIPIDS
POLYPHENOLS
PORTAL SYSTEM
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
TISSUE DISTRIBUTION
VEINS
VERTEBRATES