Study of in vitro and in vivo stability of liposomes loaded with calcitonin or indium in the gastrointestinal tract

Journal Article · Thu Dec 31 23:00:00 EST 1992 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(93)90573-L· OSTI ID:5645429

Arieen, A; Goigoux, C; Baquey, C; Dupuy, B ^[1]

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1. Universite de Bordeaux II (France)

Factors affecting liposome transport to the blood compartment after oral administration to rats were evaluated. A high entrapment of calcitonin (CT) was obtained when the vesicles were prepared by sonication and were composed of egg phosphatidylcholine, cholesterol and stearylamine. In vitro tests showed that the liposomes were stable in light acidic or basic buffers, but that they were partly lysed in pH 2.5, 10 mM bile salts and pancreatin. Oral administration of liposomes entrapping calcitonin in fasting rats showed that the vesicles facilitate transport of the hormone to the general circulation and that they increase the lifetime of [sup 125]I-CT in blood. Oral administration of liposomes entrapping radioactive indium in fasting rats did not induce radioactivity in blood. This could be explained by disruption of most of the vesicles in the enterocytes.

• OSTI ID: 5645429
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 53:16; ISSN 0024-3205; ISSN LIFSAK
• Country of Publication: United States
• Language: English

Related Subjects

560160* -- Radionuclide Effects
Kinetics
& Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CALCITONIN
DIGESTIVE SYSTEM
ELEMENTS
ENCAPSULATION
GASTROINTESTINAL TRACT
HORMONES
IN VITRO
IN VIVO
INDIUM
LIPOSOMES
METALS
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIONUCLIDE KINETICS
STABILITY