Biological effects of DNA repair, including mutagenesis: Progress report, 1 April 1988--31 March 1989

Technical Report · Sat Apr 01 04:00:00 EST 1989

DOI:https://doi.org/10.2172/6278443· OSTI ID:6278443

Hutchinson, H.

The major purpose of the research on this project is to study at the molecular level the gross rearrangements that occur in the DNA of mammalian cells, particularly those rearrangements induced by the action of genotoxic agents such as ionizing radiation. The cells being studied are Chinese Hamster Ovary (CHO)-K1 cells in which a piece of plasmid DNA has been stably incorporated into a chromosome. The piece of plasmid DNA contains both the Escherichia coli gpt gene and a neo gene, separated by about two kilobases. Mutations involving the gpt gene are identified by resistance of the cells to 6-thioguanine. Deletions can be detected quickly by loss of the neo gene (loss of resistance to G418). More information about the deletions, and information on other types of rearrangements, can be obtained by Southern blots, using plasmid DNA as a hybridization probe. 5 refs.

• Research Organization: Yale Univ., New Haven, CT (USA). Dept. of Molecular Biophysics and Biochemistry
• DOE Contract Number: FG02-87ER60521
• OSTI ID: 6278443
• Report Number(s): DOE/ER/60521-2; ON: DE89010018
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BACTERIA
CELL CONSTITUENTS
CHO CELLS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
ESCHERICHIA COLI
GAMMA RADIATION
GENE MUTATIONS
HYBRIDIZATION
IONIZING RADIATIONS
MICROORGANISMS
MIXING
MODIFICATIONS
MOLECULAR BIOLOGY
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLASMIDS
PROGRESS REPORT
RADIATIONS
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES