Biological effects of DNA repair, including mutagenesis
Technical Report
·
OSTI ID:5543788
The research performed under this grant was a study of gross rearrangements induced by ionizing radiation in the DNA of Escherichia coli and Chinese hamster ovary (CHO) cells. In E. coli, lysogens were constructed with lambda prophage containing lacZ and tet genes surrounded by 100 kilobases of nonessential DNA. Mutations induced by gamma rays were more than 10% large deletions between 0.5 and 70 kilobases in size, all centered on a ColEl origin of DNA replication also located on the prophage. This origin is needed for radiation induced deletions. Many deletions induced in mammalian cells by ionizing radiation may be centered on the many origins of replication that are not essential for cell viability PL61 was constructed with adjacent gpt and neo genes to facilitate study of radiation-induced deletions. Although this cell line was very useful for the study of point mutations induced by various agents in gpt, it was not useful for studies of deletions or insertions because of rearrangements that occured during the process of rescuing the DNA from the genome. 12 refs.
- Research Organization:
- Yale Univ., New Haven, CT (United States). Dept. of Molecular Biophysics and Biochemistry
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-87ER60521
- OSTI ID:
- 5543788
- Report Number(s):
- DOE/ER/60521-3; ON: DE91015054
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560130 -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BACTERIA
BACTERIOPHAGES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CHEMISTRY
CHO CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ESCHERICHIA COLI
GAMMA RADIATION
HYBRIDIZATION
IONIZING RADIATIONS
MICROORGANISMS
MOLECULAR BIOLOGY
MUTAGENS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLASMIDS
PROGRESS REPORT
RADIATION EFFECTS
RADIATIONS
RECOMBINANT DNA
VIRUSES
Cells
& Tissue Culture
560130 -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BACTERIA
BACTERIOPHAGES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CHEMISTRY
CHO CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ESCHERICHIA COLI
GAMMA RADIATION
HYBRIDIZATION
IONIZING RADIATIONS
MICROORGANISMS
MOLECULAR BIOLOGY
MUTAGENS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLASMIDS
PROGRESS REPORT
RADIATION EFFECTS
RADIATIONS
RECOMBINANT DNA
VIRUSES