Biological effects of DNA repair, including mutagenesis: Progress report for the period June 1, 1987-March 31, 1988

Technical Report · Thu Mar 31 23:00:00 EST 1988

OSTI ID:5168958

Hutchinson, F

A study shows the effects of gamma radiation on inducing gross rearrangements in the DNA of mammalian cells in culture is described. A line of CHO cells, K1-X3/5, in which most or all of the single copy of the endogenous hprt gene on the X chromosome has been deleted was used. Various transformed cell lines have been made in which a single copy of the E. coli gpt gene has been stably incorporated into the chromosome. These cell were irradiated with gamma rays, and gpt mutants isolated by the same procedures routinely used to isolate hprt mutants. Those mutants that contain DNA with altered Southern blots are assumed to have gross rearrangements in the DNA.

• Research Organization: Yale Univ., New Haven, CT (USA). Dept. of Molecular Biophysics and Biochemistry
• DOE Contract Number: FG02-87ER60521
• OSTI ID: 5168958
• Report Number(s): DOE/ER/60521-T1; ON: DE88008359
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BACTERIA
CHO CELLS
CLONING
DNA
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
ESCHERICHIA COLI
GAMMA RADIATION
GENE MUTATIONS
IONIZING RADIATIONS
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROGRESS REPORT
RADIATIONS
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES